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For differential expression analysis using the R package limma (27) (see below, section Comparison of group means), 2 parameters estimated by RUVs were included as covariates in the linear model, while the normalized logCPM data without RUVs correction was used as expression matrix input, as is recommended by the authors of the RUVSeq package for this type of analysis
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Isoginkgetin, a natural bioflavonoid with antioxidant and immunomodulatory activities, has been shown to alleviate LPS-induced ferroptosis in pulmonary epithelial cells and improve the M1/M2 polarization imbalance in LPS-stimulated macrophages via HOXA5-mediated inhibition of the TLR4/NF- signaling pathway, thereby exerting protective effects in S-ALI in BEAS-2B cells (Lu et al., 2025)
